Journal: Journal of Muscle Research and Cell Motility

Article Title: Protein phosphatase 2A affects myofilament contractility in non-failing but not in failing human myocardium

doi: 10.1007/s10974-011-9261-x

Figure Lengend Snippet: NF myocardium ( n = 2) was treated with 0U, 0.5U or 5U PP2A for 40 min at RT. Subsequently, proteins were separated by 1D gel electrophoresis ( a ). Phosphorylation signals of all proteins on ProQ Diamond-stained gels were divided by their SYPRO signals to correct for minor differences in loading. PKA-dependent bis-phosphorylation of cTnI at Ser23/24 was measured by Western blot analysis and normalized to α-actinin ( b ). cTnT troponin T, MLC-2 myosin light chain 2, MHC myosin heavy chain, MLC-1 myosin light chain 1, MW molecular weight marker

Article Snippet: Alternatively, the inhibition of PP2A C has been shown to minimize the metabolic damage of LV tissue in failing myocardium (Weinbrenner et al. ).

Techniques: Nucleic Acid Electrophoresis, Phospho-proteomics, Staining, Western Blot, Molecular Weight, Marker

Journal: Journal of Muscle Research and Cell Motility

Article Title: Protein phosphatase 2A affects myofilament contractility in non-failing but not in failing human myocardium

doi: 10.1007/s10974-011-9261-x

Figure Lengend Snippet: Homogenates from NF and failing (ICM; DCM) RV and LV myocardium were solubilized in a SDS-buffer and then separated electrophoretically. The immunological detection of PP2A subunits was performed by use of specific antibodies directed against Cα/β ( a ), B56α ( b ), and Aα ( c ). The statistical summarization of quantification is depicted as PhosphorImager units. Signals were standardized to calsequestrin (CSQ). * P < 0.05 vs. NF; + P < 0.05 vs. RV

Article Snippet: Alternatively, the inhibition of PP2A C has been shown to minimize the metabolic damage of LV tissue in failing myocardium (Weinbrenner et al. ).

Techniques: